| I. Introduction
Aleutian Disease Virus (ADV) is a persistent, highly
contagious parvovirus that affects ferrets (Mustela putorius furo) and
is characterized by the formation of non-neutralizing antibodies,
general plasmacytosis as well as gross physical and other
histopathological symptoms and signs. Age and stress can exacerbate
symptoms and may cause expression of the virus. ADV is spread via casual
contact and is present in the blood, saliva, feces and urine of infected
ferrets. ADV Antibody can be detected approximately 2 weeks after
infection. Ferrets found to be positive for ADV Antibody should be
isolated as suspected carriers of the disease.
II. Principle of the Test
An oral fluid (saliva) sample is added to an absorbent
pad in the bottom of the QuickChek™ ADV device and migrates by capillary
action onto a sample pad contained within the device. Any ADV antibodies
present in the sample will bind to a specific ADV-related protein coated
onto a "carrier" material (red colored) contained within the sample pad
to form the conjugate. The TEST area (designated by a T on the edge of
the plastic device) contains a second binding protein that will
immobilize any ADV Antibody-conjugate as it moves through the device. A
red line in this region signifies a POSITIVE test. A red line in
the CONTROL area (designated by a C) signifies the test is functioning
properly. A red line in the control (C) area but no color in the test
(T) area signifies a NEGATIVE test for ADV Antibody.
QuickChek™ ADV is similar to the ADV Antibody ELISA
test but is formatted as a point-of-care test (POCT) for ease of use in
veterinary clinics and hospitals, at shelters and at home.
III. Contents of the QuickChek™ Test
Kit
(1) QuickChek™ ADV test
(1) Oral Fluid (Saliva) Collection Swab
(1) Plastic Tube with Collection Fluid
(1) Applicator Pipet
IV. Storage and Stability
Store the QuickChek™ ADV test at room temperature
(15-30° C). Do not freeze. QuickChek™ ADV is stable until the expiration
date. |
